RNA-Seq of Drosophila willistoni: testis - SRA

SRX12281176: RNA-Seq of Drosophila willistoni: testis
1 ILLUMINA (Illumina HiSeq 2000) run: 32.7M spots, 4.6G bases, 1.8Gb downloads

Design: Virgin adult males and females were collected and aged 6-10 days before dissecting out testes,ovaries and accessory glands. All attached ducts were removed from the gonad tissue. Tissues were stored in RNAlater (Ambion) at -20C until RNA extraction. Immediately before RNA extraction RNAlater was removed by adding 1 mL RNase-free phosphate-buffered saline (PBS), centrifuging the sample 5,000 x g at 4 degrees C for 5 minutes, replacing the supernatant with 1 mL fresh PBS, centrifuging again, and replacing the supernatant with extraction buffer (XB) from the Arturus(R) PicoPure (R) kit according to the manufacturer's instructions. We included an on-column DNase digestion in the RNA extraction protocol.Libraries were constructed following the Illumina TruSeq Stranded mRNA Sample Prep Kit with oligo-dT selection. The resulting cDNA fragments were sheared to ~120-200 bp fragments. These fragments were used for adapter ligation and sequencing.

Submitted by: USP Universidade de Sao Paulo

Study: Ovary, Testis and Accessory Gland sequencing of Drosophila species

PRJNA764902 • SRP338066 • All experiments • All runs

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Purpose: Sequencing of gonads of Drosophila willistoni species for identification of sex biased genes and other purposes.Methods: Virgin flies were collected and aged 6 to10 days before dissecting 2 to 3 replicates of testes or ovaries. Total RNA was extracted using the Arcturus PicoPure kit . Illumina TruSeq RNA library kits were used to poly A select and reverse-transcribe mRNA, shear cDNA into 150 bp fragments, and produce libraries for paired end 70 bp sequencing on a HiSeq2000. Real Time Analysis v1.13 from Illumina module processed images, called bases, and provided base qualities.

Sample:

SAMN21533501 • SRS10255123 • All experiments • All runs

Organism: Drosophila willistoni

Library:

Name: Dwil_T1

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 32.7M spots, 4.6G bases, 1.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR15992686	32,691,989	4.6G	1.8Gb	2021-09-21

ID:: 16200949

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