show Abstracthide AbstractHeat stress is an important environmental factor affecting the growth and application of perennial ryegrass (Lolium perenne L.). Studies have shown that exogenous addition of methyl jasmonate (MeJA) can improve the heat tolerance of perennial ryegrass. In order to explore the mechanism of exogenous MeJA improving the heat tolerance of perennial ryegrass, RNA-seq was performed on perennial ryegrass control (CK), high temperature 12h (H), MeJA pretreatment (T), MeJA pretreatment + high temperature 12h (T-H) materials.