GSM1880724: sample_83; Bombus terrestris; RNA-Seq - SRA

SRX1242068: GSM1880724: sample_83; Bombus terrestris; RNA-Seq
1 ILLUMINA (Illumina Genome Analyzer) run: 7.6M spots, 762.1M bases, 492.7Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Conservation and modification of genetic and physiological toolkits underpinning diapause in bumble bee queens

PRJNA295976 • SRP063883 • All experiments • All runs

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Here, we examined the transcriptomic changes associated with diapause and CO2 treatment in B. terrestris queens before, during and post diapause and in the short and long term after CO2 treatment Overall design: Examination of transcriptomic changes in 25 queens assinged to 5 different groups: 5-10 days old mated, 5-10 days old mated right after CO2 treatment, 2.5 months old diapausing queens that were kept under 4 C for 9-10 weeks and two different egg-layer founders: 4 months old, post diapause and 1.5 months old post CO2 treatment

Sample: sample_83

SAMN04076292 • SRS1067768 • All experiments • All runs

Organism: Bombus terrestris

Library:

Instrument: Illumina Genome Analyzer

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Queens sampled were flash frozen with dry ice and were immediately transfer to -80°C until further analyses. Eviscerated abdomens with attached fat bodies from individual bees were dissected under dry ice. Total RNA samples were extracted from the abdominal fat bodies using the RNeasy mini kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. RNA quantity and quality were assayed with a ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington DE) and a 2100 Bioanalyzer (Agilent, Santa Clara, CA). RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM1880724

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 7.6M spots, 762.1M bases, 492.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR2396657	7,621,052	762.1M	492.7Mb	2015-10-05

ID:: 1789135

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