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SRX1333570: De novo assembly and transcriptome characterization of major growth-related genes of Penaeus monodon in different tissues
1 ILLUMINA (Illumina MiSeq) run: 23.2M spots, 6.4G bases, 3.8Gb downloads

Submitted by: Institute of Biotechnology, Vietnam Academy of Sci
Study: Penaeus monodon Transcriptome or Gene expression
show Abstracthide Abstract
Black tiger shrimp (Penaeus monodon) is an economically crucial species in Vietnam; however, knowledge of its transcriptome is scarce. In this study, we sequenced four transcriptome libraries from mRNA of heart, muscle, hepatopancreas and eyestalk tissue of P.monodon individuals by using Illumina MiSeq® platform and produced 78,575,346 raw pair-end reads. After quality controlling, a total of 62,327,726 pair-end reads were de novo assembled into 69,089 unigenes with an average size of 447.89bp, N50 of 481bp and transcriptome size of 30.94Mb. The assembled unigenes were compared against five public protein databases and identified 165 unigenes related to growth development. There were 14,229 differentially expressed unigenes determined by using differential expression analysis. Additionally, 12,768 simple sequence repeats (SSRs) were detected from 11,173 unigenes. These results also may bring insight to P.monodon transcriptome and would be useful for trait mapping, marker-assisted breeding, studying of population genetics and genomics and annotating on functional genomics.
Sample: Eyestalk tissue
SAMN04161439 • SRS1113517 • All experiments • All runs
Organism: Penaeus monodon
Library:
Instrument: Illumina MiSeq
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: unspecified
Layout: PAIRED
Spot descriptor:
forward151  reverse

Runs: 1 run, 23.2M spots, 6.4G bases, 3.8Gb
Run# of Spots# of BasesSizePublished
SRR264330523,217,8326.4G3.8Gb2015-10-16

ID:
1903769

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