F0malebrainH1 - SRA

SRX1406655: F0malebrainH1
1 ILLUMINA (Illumina MiSeq) run: 5.8M spots, 1.7G bases, 921.5Mb downloads

Design: libraries of female and male’s brains were constructed (2 X 2 for the normoxic group, 2 X 2 for the hypoxic group) using the TruSeq Stranded RNA LT Sample Prep Kit (Illumina), each prepared from 300 ng of total RNA. The cDNA libraries were prepared according to manufacturer’s instruction as previously described [13]. Briefly, Index codes were ligated to identify individual samples. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads (Illumina, San Diego, USA), and was then fragmented using divalent cations in the Illumina fragmentation buffer under 94 ?C for 1 min. First and second strand cDNAs were synthesized using random oligonucleotides and SuperScript II, followed by DNA polymerase I and RNase H. Overhangs were blunted by using exonuclease/polymerase and, after 3’ end adenylation, Illumina PE adapter oligonucleotides were ligated. DNA fragments that ligated with adaptor molecules on both ends were enriched using the Illumina PCR Primer Cocktail in a 15-cycle PCR reaction. Products were purified and quantified using the AMPure XP and the Agilent Bioanalyzer 2100 systems, respectively. Then the concentration of libraries was quantified using KAPA Library Quantification Kits. Paired-end reads, each of 150 bp read-length, were sequenced on the Illumina MiSeq sequencer.

Submitted by: The Chinese University of Hong Kong

Study: Oryzias melastigma brain transcriptome HNF0

PRJNA300722 • SRP065579 • All experiments • All runs

Sample:

SAMN04229043 • SRS1143218 • All experiments • All runs

Organism: Oryzias melastigma

Library:

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Spot descriptor:

1 forward151 reverse

Runs: 1 run, 5.8M spots, 1.7G bases, 921.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR2886795	5,795,333	1.7G	921.5Mb	2016-11-01

ID:: 1992998

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