GSM1942563: SUS1_1; Cricetulus griseus; RNA-Seq - SRA

SRX1436209: GSM1942563: SUS1_1; Cricetulus griseus; RNA-Seq
1 ILLUMINA (Illumina MiSeq) run: 17.7M spots, 2.4G bases, 1.6Gb downloads

Submitted by: NCBI (GEO)

Study: Revealing suspension adapation of CHO cell in serum free media

PRJNA302601 • SRP066355 • All experiments • All runs

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Purpose : We performed strand-specific RNA-seq analysis of CHO cells according to adaptation trajectory to identify the specific genes responded to the suspension adaptation. Result : Cell growth, replication, and nucleotide metabolism categories were enriched in up regulated clusters whereas cellular community category which contains focal adhesion related genes were enriched in down regulated clusters Overall design: CHO-K1 mRNA profiles during suspension adaptation generated by RNA sequencing, in duplicate, using Illumina MiSeq.

Sample: SUS1_1

SAMN04275176 • SRS1167177 • All experiments • All runs

Organism: Cricetulus griseus

Library:

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was harvested using Trizol reagent.rRNA was specifically removed using Ribo-Zero kit (Epicentre, Madison, WI). TruSeq Stranded mRNA LT Sample Prep Kit (Illumina Inc., San Diego, CA) was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina Inc., San Diego, CA) according to manufacturer's instructions

Experiment attributes:

GEO Accession: GSM1942563

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 17.7M spots, 2.4G bases, 1.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR2922599	17,682,443	2.4G	1.6Gb	2015-11-22

ID:: 2031545

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