RNA-seq of mytilus californianus: adult gill - SRA

SRX14387848: RNA-seq of mytilus californianus: adult gill
1 ILLUMINA (Illumina HiSeq 2500) run: 9.5M spots, 2.8G bases, 1.2Gb downloads

Design: RNA was extracted from 0.04 g of gill tissue by grinding it in a mortar/pestle in liquid nitrogen with TRI reagent (Sigma), quantified by UV-Vis spectroscopy (Implen Nanophotometer), and cleaned with the Qiagen RNeasy Mini Plus Kit following the manufacturers protocol (Qiagen, MD, USA). Small fragment cDNA library construction, barcoding, and 150 bp paired-end (PE150; 12 samples used to generate the de novo transcriptome) or 50 bp paired-end (PE50; 49 samples from 5 treatments used to quantify expression levels) Illumina HiSeq 2500 rapid-run sequencing was performed by the Genome and Cytometry Core at the University of Southern California. The average fragment length and insert sizes were 350bp (PE150) and 380bp (PE50).

Submitted by: Chapman University

Study: Mussel Mytilus californianus transcriptome and proteome

PRJNA800777 • SRP360224 • All experiments • All runs

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The objective of this study was to investigate the role of differential gene and protein expression in acclimatization of the bivalve rocky intertidal mollusk Mytilus californianus to distinct intertidal habitats. We exposed adult M. californianus to various field and lab treatments that vary in degrees of environmental stress and then performed both RNAseq and proteomics analysis to gain a full understanding of which genes and proteins have altered expression in these unique microhabitats, and whether this varies across individuals.

Sample:

SAMN25282678 • SRS12028610 • All experiments • All runs

Organism: Mytilus californianus

Library:

Name: 4e_EFA_ref

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PolyA

Layout: PAIRED

Runs: 1 run, 9.5M spots, 2.8G bases, 1.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR18246431	9,473,688	2.8G	1.2Gb	2022-03-30

ID:: 20440115

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