GSM1969524: F1; Paralichthys olivaceus; RNA-Seq - SRA

SRX1474056: GSM1969524: F1; Paralichthys olivaceus; RNA-Seq
2 ILLUMINA (Illumina HiSeq 2000) runs: 26.9M spots, 5.4G bases, 2.6Gb downloads

Submitted by: NCBI (GEO)

Study: Gonadal transcriptome analysis in sterile double haploid Japanese flounder

PRJNA305624 • SRP067231 • All experiments • All runs

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We used the Illumina sequencing platform to analyze the transcriptome of sterile female DH Japanese flounder in order to identify major genes that cause sterility and to provide a molecular basis for an intensive study of gonadal development in teleosts. Through sequencing, assembly, and annotation, Overall design: Three sterile female japanese flounders were control samples and three fertile ones were references samples

Sample: F1

SAMN04330078 • SRS1200124 • All experiments • All runs

Organism: Paralichthys olivaceus

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Gonads were removed, flash frozen in liquid nitrogen, and RNA was harvested using RNeasy Mini Kit.The cDNA libraries were constructed for each pooled RNA sample using the TruSeqTM RNA sample preparation kit (Illumina, Inc.) according to the manufacturer’s instructions. The tagged cDNA libraries were pooled in equal ratio and used for 101 bp paired-ends sequencing with an Illumina HiSeqTM 2000 RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM1969524

Links:

NCBI link: NCBI Entrez (gds)

Runs: 2 runs, 26.9M spots, 5.4G bases, 2.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR2985181	3,990,883	798.2M	510.7Mb	2015-12-15
SRR2985182	22,873,122	4.6G	2.1Gb	2015-12-15

ID:: 2084398

SRA

Sequence Read Archive

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