RNA-seq of Cyprinodon: embryonic head - SRA

SRX1478919: RNA-seq of Cyprinodon: embryonic head
1 ILLUMINA (Illumina MiSeq) run: 1.9M spots, 929M bases, 561.4Mb downloads

Design: Total RNA was extracted from dissected heads of full-sib F1 lab reared embryos, larvae, or juvenile C. variegatus produced by breeding a single wild caught male and wild caught female. Heads were stored in RNA-later prior to RNA extraction. RNA was extracted using an ambion TRIzol Plus RNA Purification Kit. Illumina libraries were prepared for sequencing using an NEBNext Ultra Directional RNA Library Prep Kit for Illumina with NEBNext poly(A) magnetic beads for mRNA isolation. RNA was chemically fragmented following the NEB protocol, except that the time was reduced to produce insert sizes of approx. 250bp. Random primers were used to produce cDNA.

Submitted by: Cornell University

Study: RNAseq of Cyprinodon pupfishes

PRJNA305422 • SRP067182 • All experiments • All runs

show Abstracthide Abstract

Understanding the evolution of novel morphologies is fundamental to the study of evolution. Illumina RNAseq was used to investigate gene expression divergence and sequence divergence among incipient species of pupfish that vary in skull morphology.

Sample:

SAMN04327328 • SRS1204650 • All experiments • All runs

Organism: Cyprinodon variegatus

Library:

Name: Cvar_ss_32_miseq

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM PCR

Layout: PAIRED

Runs: 1 run, 1.9M spots, 929M bases, 561.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR2991005	1,850,608	929M	561.4Mb	2015-12-15

ID:: 2089261

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