GSM1973611: Glycerol control; Strongylocentrotus purpuratus; RNA-Seq - SRA

SRX1485315: GSM1973611: Glycerol control; Strongylocentrotus purpuratus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 27.7M spots, 4.1G bases, 2.4Gb downloads

Submitted by: NCBI (GEO)

Study: Gene expression alterations in SoxC knockdown sea urchin embryos

PRJNA306145 • SRP067439 • All experiments • All runs

show Abstracthide Abstract

Transcription factor SoxC is required for all neural development in purple sea urchin S. purpuratus embryos. To begin to develop a gene regulatory network for neural development, we used RNA-Seq to compare transcript populations in SoxC knockdown and control embryos. Overall design: SoxC function was knocked down by morpholino oligo injection. RNA from about 1000 embryos were collected for both control and knockdown samples.

Sample: Glycerol control

SAMN04346832 • SRS1208630 • All experiments • All runs

Organism: Strongylocentrotus purpuratus

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: After 30 hours of development, total RNA was extracted with the NucleoSpin RNAII kit from Mcherey-Nagel GmbH.

Experiment attributes:

GEO Accession: GSM1973611

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 27.7M spots, 4.1G bases, 2.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR3017856	27,655,945	4.1G	2.4Gb	2015-12-18

ID:: 2095657

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