Design: For RNA-seq, tissue samples were taken from a multiple adult Brienomyrus brachyistius obtained through the pet trade through unknown locations (BB1, BB100 BB2, BB3, BB36, BB4, BBN22, BB-OE, BBR, BBY1, BBY2, BBY3, BBY4. For each specimen, individuals were identified as Brienomyrus brachyistius morphologically and EOD for all specimens was P0-present). Tissues sampled were: brain, kidney, electric organ, skeletal muscle, the skin along the flank (unenriched for electroreceptors), the skin of the face (enriched for electroreceptors), gonadal tissue, gill, spleen, swim bladder. In addition, samples of knollenorgans, mormyromasts and bare skin were obtained with assistance from Dr. Carl Hopkins. Each tissue sample was dissected freshly into RNAlater, stored at 4C overnight, and then transferred to -20C until RNA isolation. RNA-isolation was performed using an RNA-easy Mini Kit for lipid tissues (Qiagen). Extractions for knollenorgans, mormyromasts and skin were performed using a standard Trizol extraction. Quality and concentration were assessed using a spectrophotometer and Qubit. RNA integrity was assessed using a BioAnalyzer (Agilent), all RNA samples sequenced had a RIN score >8 . Sequencing libraries were prepared using Illumina TruSeq Stranded mRNA Library Prep Kit LT.
Submitted by: Michigan State University
Study:
Brienomyrus brachyistius transcriptome sequencingshow Abstracthide AbstractSequencing of the Brienomyrus brachyistius somatic transcriptome for the purposes of genome annotation.
Sample:
Brienomyrus brachyistius RNA-Seq of Chin Skin tissue: Fish ID -BBY4Library:
Name: BBY4_Chin_5
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: Oligo-dT
Layout: PAIRED
Runs:
1 run, 4M spots, 608M bases, 377.2Mb