GSM2065737: Rep_3 6 WL; Setaria viridis; RNA-Seq - SRA

SRX1590968: GSM2065737: Rep_3 6 WL; Setaria viridis; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 12.4M spots, 2.5G bases, 1.4Gb downloads

Submitted by: NCBI (GEO)

Study: Grasses suppress shoot-borne roots to conserve water during drought

PRJNA312459 • SRP070487 • All experiments • All runs

show Abstracthide Abstract

Setaria viridis, the wild ancestor of millet, exhibits strong repression of crown root growth in drought. We compare in gene expression in the S. viridis crown between drought vs watered treatments. Overall design: RNA from Lower region (crown) or Upper region (stem) of watered (W) or drought (D) treated Setaria plants were harvested at 6 or 9 days after sowing; there are 8 samples per biological repeat, 3 biological repeats.

Sample: Rep_3 6 WL

SAMN04502934 • SRS1304879 • All experiments • All runs

Organism: Setaria viridis

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was purified by Zymo Plant RNA kit Ribosomal RNA was depleted by Ribozero Plant Root/Seed kit, strand-specific libraries were made using NEBNext Ultra Directional kit for Illumina; libraries were amplified with 6 bp barcoded pcr primers 12 libraries were pooled per lane; aimed for 10 million reads per library.

Experiment attributes:

GEO Accession: GSM2065737

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 12.4M spots, 2.5G bases, 1.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR3176673	12,395,980	2.5G	1.4Gb	2016-07-25

ID:: 2245490

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