Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was purified by Zymo Plant RNA kit Ribosomal RNA was depleted by Ribozero Plant Root/Seed kit, strand-specific libraries were made using NEBNext Ultra Directional kit for Illumina; libraries were amplified with 6 bp barcoded pcr primers 12 libraries were pooled per lane; aimed for 10 million reads per library.