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SRX1605655: GSM2076236: Kai_Jul; Tursiops truncatus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 24.5M spots, 3.1G bases, 1.3Gb downloads

Submitted by: NCBI (GEO)
Study: RNA-seq analysis of seasonal and individual variation in blood transcriptomes of healthy captive bottlenose dolphins
show Abstracthide Abstract
Four captive bottlenose dolpins housed in Kona, Hawaii, were serially sampled at approximately monthly intervals over the course of one year, in order to establish baseline information on the content and variation of the dolphin blood transcriptome. Overall design: Illumina based RNA-seq analyses were carried out on globin-depleted RNA from blood using the Ensembl dolphin genome as a guide. The data set was interrogated for transcripts exhibiting circa-annual rhymthicity, differential expression by season or sex, or correlation to hematological parameters
Sample: Kai_Jul
SAMN04523306 • SRS1315300 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Whole blood RNA was extracted using a PAXgene Blood RNA Kit (Qiagen). Total RNA samples were globin-reduced using an Rnase H assay targeting HBA, HBB, and HBM transcripts. Libraries were constructed using a NEBNext ultra Directional RNA Library Prep kit for Illumina and indexed with the NEBNextMultiplex Oligos for Illumina
Experiment attributes:
GEO Accession: GSM2076236
Links:
Runs: 1 run, 24.5M spots, 3.1G bases, 1.3Gb
Run# of Spots# of BasesSizePublished
SRR319509524,515,2063.1G1.3Gb2016-09-12

ID:
2295716

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