Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Whole blood RNA was extracted using a PAXgene Blood RNA Kit (Qiagen). Total RNA samples were globin-reduced using an Rnase H assay targeting HBA, HBB, and HBM transcripts. Libraries were constructed using a NEBNext ultra Directional RNA Library Prep kit for Illumina and indexed with the NEBNextMultiplex Oligos for Illumina