Ruveia_undamaged - SRA

SRX1744808: Ruveia_undamaged
1 LS454 (454 GS FLX Titanium) run: 146,765 spots, 79.8M bases, 178.5Mb downloads

Design: Total RNA was extracted from 200 mg of olive mesocarp with RNeasy Plant Mini Kit (Qiagen Hilden, Germany) and treated with DNase I (Ambion, Austin, TX, USA). Three biological replicates consisting of 5 olives each were used for each biological condition (control undamaged olives). cDNAs were synthesized using the SMART PCR cDNA Synthesis kit (Clontech, Palo Alto, CA, USA). First strand synthesis was performed using 8 µg of total RNA, as described by Alagna et al. (2009). Double stranded cDNAs were purified using the QIAquick PCR purification kit (Qiagen, Hilden, Germany) and quantified with a fluorimeter (Victor 2, Perkin Elmer, Wellesley, MA, USA). To verify the cDNA quality and fragment length, all samples were separated on a 1.5% agarose gel.

Submitted by: ENEA

Study: Olea__bactrocera

PRJNA320812 • SRP074450 • All experiments • All runs

show Abstracthide Abstract

Pyrosequencing of four cDNA libraries from damaged (pool of different stages of fly attack) and control undamaged fruits of ‘Ortice’ and ‘Ruveia’ (Olea europaea L.), characterized by a different tolerance to Bactrocera oleae, generated a comprehensive repertory of transcripts useful to study the genes modulated by Bactrocera oleae attack.

Sample: Ruveia_undamaged

SAMN04957804 • SRS1423869 • All experiments • All runs

Organism: Olea europaea

Library:

Instrument: 454 GS FLX Titanium

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: unspecified

Layout: SINGLE

Spot descriptor:

5 forward

Runs: 1 run, 146,765 spots, 79.8M bases, 178.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR3479026	146,765	79.8M	178.5Mb	2017-05-06

ID:: 2502325

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