RNA-seq of Alligator mississippiensis: gonad of embryo incubated at F... - SRA

SRX1803187: RNA-seq of Alligator mississippiensis: gonad of embryo incubated at FPT for 3 days
1 ILLUMINA (Illumina HiSeq 2000) run: 36.6M spots, 7.4G bases, 4.1Gb downloads

Design: We incubated eggs at female-producing temperature (FPT, 30.0 deg C) until developmental stage 19. Then, we increased the incubation temperature of half of the eggs to male-producing temperature (MPT, 33.5 deg C) and incubated at both temperatures. We harvested eggs at either stage 19 or after 3 or 30 days of incubation after stage 19. We subdissected the gonad, adrenal, and mesonephros tissues and performed RNA-seq on each.

Submitted by: University of California, Santa Cruz

Study: Alligator mississippiensis embryonic gene expression

PRJNA322197 • SRP075819 • All experiments • All runs

show Abstracthide Abstract

The American alligator, like all other crocodilians and many other reptiles, undergoes temperature-dependent sex determination, in which the incubation temperature of an egg determines its eventual sex. In this study, we sequence transcriptomes from gonad, adrenal, and mesonephros tissues of alligator embryos incubated at different temperatures for different lengths of time.

Sample: gonad tissue from A. mississippiensis embryo incubated at 30.0 deg C (female-producing temperature) for three days after developmental stage 19

SAMN05072561 • SRS1469507 • All experiments • All runs

Organism: Alligator mississippiensis

Library:

Name: 10_G_3d_F

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 36.6M spots, 7.4G bases, 4.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR3593719	36,560,902	7.4G	4.1Gb	2016-12-07

ID:: 2578885

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