Anterior kidney sample 12 - Day 2 - Control - SRA

SRX1853013: Anterior kidney sample 12 - Day 2 - Control
1 ILLUMINA (Illumina Genome Analyzer II) run: 3.1M spots, 457.6M bases, 308.6Mb downloads

Design: The aim of this study was to determine the transcriptomic responses in the kidney for water-borne exposure to diclofenac, one of the most widely prescribed non-steroidal anti-inflammatory drugs (NSAID) worldwide, in a model fish species (fathead minnow, Pimephales promelas). RNA was isolated from the anterior kidney of adult male fathead minnows using Tri Reagent, according to the manufacturers instructions. cDNA libraries were prepared using Illumina TruSeq RNA Sample Preparation Kit and subsequent cluster generation using TruSeq Paired-End Cluster Generation kit (Illumina, San Diego CA, USA). In total 24 samples were prepared for sequencing: 6 replicate samples from each of 4 treatment groups (0 ľgL-1 diclofenac (control) at 2 and 21 days exposure, and 25.0 ľg/L diclofenac at 2 and 21 days exposure). 8 samples were sequenced per lane using an Illumina GAII platform (76bp paired-end).

Submitted by: University of Exeter

Study: Uptake and effects on the kidney of Diclofenac exposed via the water in a model fish species.

PRJNA326109 • SRP076715 • All experiments • All runs

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Diclofenac, one of the most widely prescribed non-steroidal anti-inflammatory drugs (NSAID) worldwide, has been proven to cause adverse effects in vultures with major environmental consequences. Diclofenac is frequently detected in surface waters; however, whether this pharmaceutical poses a risk to the aquatic environment remains a debated subject and is currently unclear. We aimed to evaluate the uptake, and tissue and molecular responses in the kidney for water borne exposure to diclofenac in a model fish species (fathead minnow, Pimephales promelas).

Sample: Anterior kidney sample 12 - Day 2 - Control

SAMN05258328 • SRS1510160 • All experiments • All runs

Organism: Pimephales promelas

Library:

Name: Control_Day_2.12

Instrument: Illumina Genome Analyzer II

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 3.1M spots, 457.6M bases, 308.6Mb

Run	# of Spots	# of Bases	Size	Published
SRR3680179	3,134,048	457.6M	308.6Mb	2017-06-16

ID:: 2647035

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