Name: GSM6900586
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA samples were extracted from the whole body of juvenile P. prolifica (n = 3 for line A and n = 3 for line B) using the RNeasy Mini Plus kit (Qiagen, Redwood City, CA, USA). For juvenile fish wholebody samples, standard double-strand RNA-seq libraries were constructed using the Illumina TruSeq RNA Library Prep Kit (Illumina, San Diego, CA, USA) and the NEXTFLEX Rapid RNA-Seq Kit for Illumina Sequencing (PerkinElmer, Hopkinton, MA, USA). To obtain information on the direction of transcription, single-strand RNA-seq libraries were prepared using the NEXTFLEX Rapid Directional RNA-Seq Kit (PerkinElmer, Hopkinton, MA, USA) and TruSeq Stranded mRNA Library Prep kit (Illumina, San Diego, CA, USA) following the manufacturer's protocols. libraries were sequenced in a 2×100 bp Paired-End (PE) setting on an Illumina HiSeq2000 machine. To characterize expression genes in the embryonic stages, total RNA samples were also extracted from early embryos at developmental stage 45 of P. prolifica line A and line B, and mRNA-seq libraries were prepared using the TruSeq RNA Library Prep kit v2 (Illumina, San Diego, CA, USA). After size check on an Agilent TapeStation 4200 using the High-Sensitive D1000 Screen Tape (Agilent Technologies, Santa Clara, CA, USA), the libraries were sequenced using a 2×125 bp PE format on an Illumina HiSeq2500 Sequencer .