Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Larvae from the common rearing tanks were transferred in small batches to the wet lab where they could be measured under a microscope camera setup. Depending on developmental stage, one to several larvae were placed on a microscope slide and euthanized with a lethal dose of MS-222. The larvae were quickly photographed, measured, and sorted by size into vials containing RNAlater® (Ambion). The length of time from the point when the larvae were removed from the water to the point of being placed in RNAlater® was under two minutes. The vials of RNAlater® were kept on ice until being placed in -20°C storage at the NMFS laboratory. RNA libraries were prepared for sequencing using standard Illumina protocols.