RNA-seq of Acropora millepora: Spawning 0730PM_Ambient_16/11/2011 - SRA

SRX2037513: RNA-seq of Acropora millepora: Spawning 0730PM_Ambient_16/11/2011
1 ILLUMINA (Illumina HiSeq 2000) run: 16.7M spots, 3.3G bases, 2.1Gb downloads

Design: "Total RNA from the coral branches was isolated by homogenizing 100 mg coral tissue in 1 ml TRIzol (Invitrogen) according to the manufacturers instructions. The RNA was then extracted once with 1 volume chloroform and precipitated in ½ volume isopropanol, washed in 1 volume of 75% ethanol and subsequently dissolved in RNase-free water. These samples were then processed through a 5 M LiCl precipitation overnight at -20º C, washed 3 times with 75% ethanol and subsequently dissolved in RNase-free water. The integrity and quality of the total RNA was assessed using a Bioanalyzer (Agilent Technology). Only the samples showing intact RNA (RNA Integrity number >8) were used for the RNA-seq analysis. The Illumina TruSeq protocol was used to prepare libraries from the RNA samples. Overall, 12 libraries of full moon and new moon samples were run on one lane in the Illumina HiSeq2000 machine using the multiplexing strategy of the TruSeq protocol"

Submitted by: Bar-Ilan University

Study: Acropora millepora Raw sequence reads

PRJNA276913 • SRP055723 • All experiments • All runs

show Abstracthide Abstract

it is presently unknown how specific environmental cues function together with endogenous clock mechanism to ensure the accurate timing of gamete release. Our results show that moonlight is an important environmental cue for mass spawning synchrony, and we describe the potential mechanisms underlying the ability of corals to sense environmental cues and trigger signalling cascades that lead to gamete release.

Sample:

SAMN05597961 • SRS1631259 • All experiments • All runs

Organism: Acropora millepora

Library:

Name: 0730PM_Ambient_16/11/2011

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: other

Layout: PAIRED

Spot descriptor:

1 forward102 reverse

Runs: 1 run, 16.7M spots, 3.3G bases, 2.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR1853192	16,721,174	3.3G	2.1Gb	2015-11-05

ID:: 2946743

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity