RNA-Seq analysis and de novo transcriptome assembly of low temperatur... - SRA

SRX206130: RNA-Seq analysis and de novo transcriptome assembly of low temperature responsive transcripts of Hevea brasiliensis
1 ILLUMINA (Illumina HiSeq 2000) run: 38.7M spots, 7.7G bases, 5.1Gb downloads

Design: Transcriptome library for sequencing was constructed according to the Illumina TruSeq RNA library protocol outlined in “TruSeq RNA Sample Preparation Guide”. Briefly, mRNA was purified from 1 microgram of intact total RNA using oligodT beads. The purified mRNA was fragmented for 4 minutes at elevated temperature (94°C) in the presence of divalent cations and reverse transcribed with Superscript II Reverse transcriptase by priming with Random Hexamers. Second strand cDNA was synthesized in the presence of DNA Polymerase I and Rnase H. The cDNA was cleaned up using Agencourt Ampure XP SPRI beads (Beckman Coulter). Illumina Adapters were ligated to the cDNA molecules after end repair and addition of A base. SPRI cleanup was performed after ligation. The library was amplified using 11 cycles of PCR for enrichment of adapter ligated fragments.

Submitted by: RUBBER RESEARCH INSTITUTE OF INDIA, KOTTAYAM 686 009, KERALA, INDIA (RUBBER RESEARCH INSTITUTE OF INDIA, KOTTAYAM 686 0)

Study: Hevea brasiliensis subsp. brasiliensis Transcriptome or Gene expression

PRJNA182078 • SRP017287 • All experiments • All runs

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De novo transcriptome analysis of abiotic stress- responsive transcripts of Hevea brasiliensis.

Sample: General Sample for Hevea brasiliensis subsp. brasiliensis

SAMN01818469 • SRS376811 • All experiments • All runs

Organism: Hevea brasiliensis subsp. brasiliensis

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: size fractionation

Layout: PAIRED

Spot descriptor:

1 forward101 reverse

Runs: 1 run, 38.7M spots, 7.7G bases, 5.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR620235	38,684,929	7.7G	5.1Gb	2013-11-01

ID:: 278713

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