H. rufescens testis RNA-seq - SRA

SRX246491: H. rufescens testis RNA-seq
2 ILLUMINA (Illumina Genome Analyzer) runs: 67.8M spots, 9.3G bases, 5.8Gb downloads

Design: We extracted mRNA from the testes of 5 small male H. rufescens, synthesized strand-specific cDNA, fragmented it, ligated adapters and amplified, then sequenced with 76-bp paired-end reads on an Illumina Genome Analyzer.

Submitted by: UNIVERSITY OF WASHINGTON (NREMC)

Study: Haliotis rufescens testis transcriptome sequencing

PRJNA191103 • SRP018917 • All experiments • All runs

show Abstracthide Abstract

Abalone is a long-studied model to study rapid evolution in fertilization. Our goal was to discover new proteins present in sperm that may be important for the evolution of sperm-egg interaction. Testis mRNA was sequenced via strand-specific cDNA in order to study the testis transcriptome of Haliotis rufescens. This transcriptome was used as a database to search mass spectra from shotgun proteomics of H. rufescens sperm.

Sample: Testis RNA-seq from the Red Abalone, Haliotis rufescens

SAMN01931999 • SRS398516 • All experiments • All runs

Organism: Haliotis rufescens

Library:

Instrument: Illumina Genome Analyzer

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PCR

Layout: PAIRED

Spot descriptor:

1 forward77 reverse

Runs: 2 runs, 67.8M spots, 9.3G bases, 5.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR768363	28,082,075	3.5G	2.1Gb	2013-04-04
SRR770266	39,673,235	5.8G	3.7Gb	2013-04-04

ID:: 337633

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