lobe_7days_Control_3 - SRA

SRX2504445: lobe_7days_Control_3
1 ILLUMINA (Illumina HiSeq 2500) run: 8.4M spots, 1.7G bases, 675.3Mb downloads

Design: The aim of this study was to determine the transcriptomic responses in the olfactory system (epithelium and lobe) for water-borne exposure to high CO2, as a proxy for ocean acidification, in European sea bass (Dicentrarchus labras). RNA was isolated from the olfactory epithelium and lobe of juvenile sea bass using RNeasy mini kit (QIAGEN), according to the manufacturers instructions. cDNA libraries were prepared using Illumina NextFlex Rapid RNA-Seq Kit and subsequent cluster generation using TruSeq PE Cluster Kit v3 - cBot HS (Illumina, San Diego CA, USA). In total 16 and 24 samples were prepared for sequencing for the olfactory lobe and epithelium, respectively: 4-6 replicate samples from each of 4 treatment groups (400 ľatm (control) at 2 and 7 days exposure, and 950 ľatm at 2 and 7 days exposure). For the epithelium and lobe 8 and 16 samples per lane, respectively, were sequenced using an Illumina GAII platform (76bp paired-end).

Submitted by: University of Exeter, UK

Study: Near-future CO2 directly impairs the olfactory system of a marine fish

PRJNA362551 • SRP097118 • All experiments • All runs

show Abstracthide Abstract

Simulated ocean acidification impairs the sensory behavior and learning of most fishes studied. Using RNAseq we show that sea bass exposed to elevated CO2 for 2 and 7 days show changes in gene expression that underlie changes in behaviour.

Sample: lobe_7days_Control_3

SAMN06242023 • SRS1929280 • All experiments • All runs

Organism: Dicentrarchus labrax

Library:

Name: lobe_7days_Control_3

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 8.4M spots, 1.7G bases, 675.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR5188524	8,422,076	1.7G	675.3Mb	2018-07-17

ID:: 3628575

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