Short-term feed deprivation alters immune status of surface mucosa in... - SRA

SRX255930: Short-term feed deprivation alters immune status of surface mucosa in channel catfish (Ictalurus punctatus)
1 ILLUMINA (Illumina HiSeq 2500) run: 18.1M spots, 3.6G bases, 2.4Gb downloads

Design: Sequencing libraries were prepared with 2.14-3.25 ug of starting total RNA and processed using the Illumina TruSeq RNA Sample Preparation Kit, as dictated by the TruSeq protocol. As per the procotol, the libraries were amplified with 15 cycles of PCR and contained TruSeq indexes within the adaptors, specifically indexes 1-6. The final, amplified library yields were 30 ul of 19.8-21.4 ng/ul with an average length of 268 base pair (bp), indicating a concentration of 110-140 nM. After KAPA quantitation and dilution, the libraries were clustered 6 per lane and sequenced on a HiSeq 2000 instrument with 100bp PE reads at the HudsonAlpha Genomic Services Lab (Huntsville, AL, USA).

Submitted by: AUBURN UNIVERSITY

Study: Ictalurus punctatus Transcriptome or Gene expression

PRJNA184724 • SRP017689 • All experiments • All runs

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What are actors controlling the host immune response in the catfish gill?

Sample: Generic sample from Ictalurus punctatus

SAMN01992457 • SRS404731 • All experiments • All runs

Organism: Ictalurus punctatus

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PCR

Layout: PAIRED

Spot descriptor:

1 forward101 reverse

Runs: 1 run, 18.1M spots, 3.6G bases, 2.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR798118	18,135,202	3.6G	2.4Gb	2014-03-26

ID:: 357989

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