RNA-seq of salivary glands from Rhipicephalus sanguineus infected wit... - SRA

SRX2574408: RNA-seq of salivary glands from Rhipicephalus sanguineus infected with Ehrlichia canis: Sap1
1 ILLUMINA (Illumina HiSeq 2500) run: 54.8M spots, 9.7G bases, 6.4Gb downloads

Design: Salivary glands (SG) were dissected from engorged female ticks collected from the infected and uninfected dogs and stored in RNAlater (Ambion) at -20ºC. Total RNA from the SG was extracted using the All-in-One Purification Kit (Norgen Biotek Corporation), following the manufacturer’s instructions. RNA concentration and integrity were evaluated by analysis of rRNA integrity using Agilent 2100 Bioanalyzer (Agilent Technologies Inc.). Libraries preparation was performed with "NEBNext Ultra Directional RNA Library Prep" kit (New England Biolabs) following manufacturer’s instructions. Briefly, magnetic beads with Oligo(dT) were used to enrich poly (A) mRNA from 1 µg of total RNA. Next, the purified mRNA was disrupted into short fragments, and double-stranded cDNA was immediately synthesised. cDNA was subjected to end-repair and adenylation, then connected with sequencing adapters. Suitable fragments, purified by size selection protocol with AMPure XP beads (Beckman Coulter), were selected as templates for PCR amplification. The final library sizes and qualities were evaluated electrophoretically using an Agilent High Sensitivity DNA kit (Agilent Technologies); the fragment size range was 300–1300 bp. Subsequently, the library was sequenced using a HiSeq 2500 sequencer (Illumina) in rapid run mode. Cluster generation was performed, followed by 2x100 cycle sequencing reads separated by a paired-end turnaround. Image analysis was performed using the HiSeq control software version 1.8.4.

Submitted by: IHMT-UNL

Study: Rhipicephalus sanguineus sialotranscriptome

PRJNA362595 • SRP098920 • All experiments • All runs

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This study concerns of a transcriptomic analysis of salivary glands of adult female Rhipicephalus sanguineus in response to Ehrlichia canis infection. Such information can be used for the selection of potential targets to develop anti-tick and pathogen transmission blocking vaccines.

Sample: Salivary glands from Rhipicephalus sanguineus infected with Ehrlichia canis

SAMN06242557 • SRS1959672 • All experiments • All runs

Organism: Rhipicephalus sanguineus

Library:

Name: SAp1

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Spot descriptor:

1 forward101 reverse

Runs: 1 run, 54.8M spots, 9.7G bases, 6.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR5270353	54,784,451	9.7G	6.4Gb	2017-02-28

ID:: 3716800

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