P.leucopus_NY_Liver_transcriptome - SRA

SRX257960: P.leucopus_NY_Liver_transcriptome
1 LS454 (454 GS FLX+) run: 116,885 spots, 61.3M bases, 140.7Mb downloads

Design: Livers, gonads and brains were immediately extracted from caught mice, rinsed with PBS, and immediately placed in RNALater* (Ambion Inc., Austin, TX) on ice before transport and storage at -80*C in the lab. Total RNA was extracted using RNaqueous* kits (Ambion, Austin, TX) or Trizol* reagent (Invitrogen, Carlsbad, CA). cDNA synthesis was carried out using the SMARTer™ cDNA synthesis kit (Clontech, Mountain View, CA) or the Roche cDNA synthesis kit (Roche Diagnostics, Indianapolis, IN). cDNA pools were sequenced with multiplex identifiers using standard 454 FLX Titanium protocols and sequenced on the 454 FLX platform.

Submitted by: CUNY - THE GRADUATE CENTER

Study: Peromyscus leucopus Transcriptome or Gene expression

PRJNA192723 • SRP020005 • All experiments • All runs

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454 sequencing of cDNA libraries from pooled Peromyscus leucopus populations in New York City.

Sample: Generic sample from Peromyscus leucopus

SAMN01991075 • SRS406145 • All experiments • All runs

Organism: Peromyscus leucopus

Library:

Name: FM_P.leucopus_Male_Female_Liver_RL9_Plate4

Instrument: 454 GS FLX+

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Spot descriptor:

5 forward

Runs: 1 run, 116,885 spots, 61.3M bases, 140.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR801147	116,885	61.3M	140.7Mb	2013-09-04

ID:: 360912

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