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SRX278615: GSM1143424: RNAseq_E10.5_rep1; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 80.3M spots, 11.9G bases, 8Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: RNA-seq in whole mouse embryos at stage E10.5
show Abstracthide Abstract
An increasing number of studies, including mutant expression profiling and comparative transcriptomic analyses, require reference RNA-seq data collections in mice. Particularly, to complement previous profiling data sets based on arrays, a full RNA-seq developmental series will be required for whole embryos. E10.5 is a key reference stage as it represents the early organogenesis stage. Here, we have performed high-throughput sequencing of total RNA form whole mice embryos at embryonic stage E10.5. Overall design: Sequencing of the total RNA of whole embryos of mouse at embryonic stage E10.5.
Sample: RNAseq_E10.5_rep1
SAMN02146467 • SRS421477 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: TRI-reagent followed by chloroform extraction, phenol:chloroform:methyl-butanol (25:24:1) extraction and propanol precipitation. RNA libraries were prepared for Illumina sequencing using the standard protocol.
Experiment attributes:
GEO Accession: GSM1143424
Links:
External link:
Runs: 1 run, 80.3M spots, 11.9G bases, 8Gb
Run# of Spots# of BasesSizePublished
SRR85193480,284,66711.9G8Gb2014-03-25

ID:
395755

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