GSM2611054: DLBB14-06; Delphinapterus leucas; RNA-Seq - SRA

SRX2794569: GSM2611054: DLBB14-06; Delphinapterus leucas; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 57.4M spots, 7.2G bases, 3.3Gb downloads

Submitted by: NCBI (GEO)

Study: RNA-seq analysis of blood transcriptomes from beluga whales in Bristol Bay, Alaska, USA

PRJNA386157 • SRP106871 • All experiments • All runs

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Whole blood was collected as part of annual health assessments of beluga whales from the healthy Bristol Bay, Alaska stock during 2012-2014. Gene expression from 24 animals (8 from each year) was analyzed to establish baseline information on the content and variation of the beluga whale blood transcriptome. Overall design: Illumina based RNA-seq analyses were carried out on RNA from blood using a transcriptome assembled de novo in Trinity. The data set was interrogated for transcripts exhibiting variablity between sexes or years.

Sample: DLBB14-06

SAMN06925945 • SRS2175700 • All experiments • All runs

Organism: Delphinapterus leucas

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Whole blood RNA was extracted using a PAXgene Blood RNA kit (Qiagen). Libraries were constructed using a NEBNext ultra Directional RNA Library Prep kit for Illumina and indexed with the NEBNextMultiplex Oligos for Illumina

Experiment attributes:

GEO Accession: GSM2611054

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 57.4M spots, 7.2G bases, 3.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR5521333	57,426,419	7.2G	3.3Gb	2017-05-15

ID:: 4028839

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Sequence Read Archive

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