GSM2633876: GFP KD control - rep3 (t1); Harpegnathos saltator; RNA-Seq - SRA

SRX2839800: GSM2633876: GFP KD control - rep3 (t1); Harpegnathos saltator; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 22.1M spots, 1.2G bases, 543.9Mb downloads

Submitted by: NCBI (GEO)

Study: Brain RNA-seq one day after knockdown of the corazonin receptor in Harpegnathos ants

PRJNA387394 • SRP107741 • All experiments • All runs

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This experiment aimed at determining the immediate (d1) transcriptional consequences of knockding down the corazonin receptor in the ant brain. Overall design: Dueling Harpegnathos individuals were removed from the transition colony and injected with corazonin receptor or GFP siRNAs to perform mRNA knockdown

Sample: GFP KD control - rep3 (t1)

SAMN07150957 • SRS2213227 • All experiments • All runs

Organism: Harpegnathos saltator

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Brains (minus optic lobes) were dissected and RNA extracted with TRIzol. DNA was removed using Turbo DNase. Each brain was used to construct a separate library (biological replicate). RNA was fragmented by boiling in presence of divalent cations. RT was performed with random hexamers and dUTP was incorporated in the 2nd strand to retain strand information. Strand-specific RNA-seq (dUTP)

Experiment attributes:

GEO Accession: GSM2633876

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 22.1M spots, 1.2G bases, 543.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR5581730	22,144,136	1.2G	543.9Mb	2017-08-11

ID:: 4077683

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