immature fruit, apparently healthy - SRA

SRX286482: immature fruit, apparently healthy
1 ILLUMINA (Illumina Genome Analyzer II) run: 28.9M spots, 4.9G bases, 2.2Gb downloads

Design: Leaf and fruit samples were collected from three types of orchard trees: symptomatic (SY) and apparently healthy (AH) in an infected orchard, and healthy trees from an HLB-free location (CO). A fourth sample type, “asymptomatic” (AS) tissues were collected from infected trees displaying symptoms elsewhere in the same tree, from the same location as the AH trees. Samples were collected in four tissues: Immature Fruits (IF) Mature Fruits (MF), Young Leaves (YL), and Mature Leaves (ML); four disease sample types from each of these, to yield 16 sample types total. A pool of 5 to 10 different leaves or fruits was collected from each of five different trees per treatment group, representing five biological replicates. RNA from the five biological replicates was equally pooled to 10 µg and then used to construct one cDNA library for each of the four HLB status categories for each tissue. cDNA library construction and high throughput sequencing. RNA from the five biological replicates was equally pooled to 10 µg and then used to construct one cDNA library for each of the four HLB status categories for each tissue. Each library was run as an independent lane on a Genome Analyzer II (Illumina, San Diego, CA) to obtain read lengths of up to 85 bp per paired end.

Submitted by: UC DAVIS

Study: Citrus sinensis Transcriptome or Gene expression

PRJNA203307 • SRP022979 • All experiments • All runs

show Abstracthide Abstract

Next-generation sequencing technology was exploited to gain deeper insight into how fruit and leaf gene networks of Citrus sinensis (sweet orange) respond to infection by Candidatus liberibacter asiaticus (CaLas), one of the causative agents of Huanglongbing disease (HLB). HLB or “citrus greening” is the most destructive citrus disease worldwide, where little is known about mechanisms of the physiological changes in infected plants.

Sample: immature fruit, apparently healthy

SAMN02152589 • SRS429285 • All experiments • All runs

Organism: Citrus sinensis

Library:

Instrument: Illumina Genome Analyzer II

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Spot descriptor:

1 forward86 reverse

Runs: 1 run, 28.9M spots, 4.9G bases, 2.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR867394	28,923,719	4.9G	2.2Gb	2015-07-22

ID:: 404372

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity