Swanson clonal line testis - SRA

SRX2894150: Swanson clonal line testis
1 ILLUMINA (Illumina Genome Analyzer IIx) run: 42.7M spots, 8.5G bases, 5.4Gb downloads

Design: RNA-Seq libraries were constructed with the mRNA Sequencing Sample Preparation Kit (Illumina). PolyA+ messenger RNA was selected from 1 ?g of RNA with magnetic oligo (dT) beads, chemically fragmented and converted to cDNA with random hexamers. Double stranded cDNAs were end-repaired, and the 3-ends were A-tailed followed by ligation of Illumina sequencing and amplification adapters randomly to the ends. The adaptor-ligated cDNAs were loaded onto 2% agarose E-gels (Invitrogen) and the fraction containing 200500 bp was excised. Size-selected cDNAs were amplified by PCR with primers that introduced unique barcodes to each library. The libraries were then quantitated with the Qubit (Life Technologies) and the average size was determined on an Agilent bioanalyzer DNA7500 DNA chip (Agilent Technologies) and diluted to 10nM. Each library was loaded onto one lane of an 8-lane flowcell for cluster formation and sequenced on an Illumina Genome Analyzer IIx according to the manufacturers protocols. The fastq files were generated with Casava version 1.6.

Submitted by: USDA/ARS

Study: Rainbow trout RNA-seq from Swanson clonal line (13 tissues) and two tissues from pooled samples

PRJNA389609 • SRP108798 • All experiments • All runs

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In this study, non-normalized cDNA libraries were sequenced from 13 different tissues of fish from the Swanson YY male doubled haploid rainbow trout and two different tissues of pooled rainbow trout from a domesticated aquaculture strain (NCCCWA). This study provides comprehensive de novo transcriptome information that is suitable for functional and comparative genomics studies in rainbow trout, including annotation of the genome.

Sample:

SAMN07203792 • SRS2261675 • All experiments • All runs

Organism: Oncorhynchus mykiss

Library:

Name: S_Testis

Instrument: Illumina Genome Analyzer IIx

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 42.7M spots, 8.5G bases, 5.4Gb

Run	# of Spots	# of Bases	Size	Published
SRR5657606	42,694,873	8.5G	5.4Gb	2017-06-09

ID:: 4144659

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