Instrument: Illumina HiSeq 1000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: 3'-seq libraries were prepared and sequenced as described (Sanfilippo P, Miura P, Lai EC: Genome-wide profiling of the 3' ends of polyadenylated RNAs. Methods 2017, 75:in press.), using 2 μg of total RNA as starting material. Total RNA-seq libraries were prepared starting with 1 μg of total RNA in water. The 3p-seq libraries were sequenced using a hiseq-1000 with SE-50 mode at the genomics core facility at MSKCC. Total RNA-seq libraries were prepared using the Illumina TruSeq Stranded Total RNA Library Prep Kit (cat. No. RS-122-2201). The protocol was followed exactly as per manufacturer's instructions. The libraries were sequenced on a hiseq-1000 sequencer at the genomics core facility at MSKCC. 3p-seq hiseq-1000 with SE-50; Total RNA-seq paired-end PE75