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SRX305633: GSM1162853: AF11-8940_650; Melitaea cinxia; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 5.1M spots, 1G bases, 630.9Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Dispersal, flight metabolism and gene expression in the Glanville fritillary butterfly
show Abstracthide Abstract
We have measured flight effect in gene expression using individual-based RNA-seq data from two regional populations of the Glanville fritillary butterfly (Melitaea cinxia). Largest number of differentially expressed genes were between populations (3840 genes) and between males and females (1622 genes). 801 genes had significant flight effect. Enriched GO and KEGG categories among these genes included hypoxia, glycolysis, and TCA cycle. Overall design: RNA-seq from thorax, 85 individuals from two populations in four groups (Flight and Control at two time points 1h and 20h). Populations ''AL'' and ''PT'' refer to two islands in Baltic Sea. ''AL'' stands for Ã...land and ''PT'' stands for Pikku Tytärsaari.
Sample: AF11-8940_650
SAMN02203636 • SRS444757 • All experiments • All runs
Organism: Melitaea cinxia
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted from frozen butterfly thorax with Trizol (Life Technologies Corporation, CA, USA) followed by chloroform, phenol-chloroform-isoamylalcohol and second chloroform purification. RNA was precipitated with isopropanol and washed with ethanol. RNA quality was verified with NanoDrop measurement (Thermo Fischer Scientific Inc., MA, USA) and Bioanalyzer run (Agilent Technologies, CA, USA). An in-house strand-specific library construction protocol based on polyA-anchoring. Two libraries with average insertsizes 450nt and 650nt were constructed for each individual.
Experiment attributes:
GEO Accession: GSM1162853
Links:
External link:
Runs: 1 run, 5.1M spots, 1G bases, 630.9Mb
Run# of Spots# of BasesSizePublished
SRR9005815,145,5961G630.9Mb2015-06-01

ID:
429202

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