GSM2735508: Gamergate brain - d120 - rep12 - tech2; Harpegnathos salt... - SRA

SRX3070759: GSM2735508: Gamergate brain - d120 - rep12 - tech2; Harpegnathos saltator; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 22.6M spots, 1.7G bases, 715.8Mb downloads

Submitted by: NCBI (GEO)

Study: Brain RNA-seq 120 days after worker-gamergate transitions in Harpegnathos ants

PRJNA327090 • SRP077502 • All experiments • All runs

show Abstracthide Abstract

Harpegnathos workers were induced to transition to reproductive gamergates and 120 days later mRNA from their brains was sequenced to determine changes in gene expressioin Overall design: Ants were removed from their original colony at d0 and placed in boxes lacking a dominant reproductive. After 120 days individuals were scored for reproductive status by inspecting their ovaries and brains (minus optic lobes) were dissected and processed for sequencing.

Sample: Gamergate brain - d120 - rep12 - tech2

SAMN07461202 • SRS2415127 • All experiments • All runs

Organism: Harpegnathos saltator

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Brains (minus optic lobes) were dissected and RNA extracted with TRIzol. DNA was removed using Turbo DNase. RNA was fragmented by boiling in presence of divalent cations. RT was performed with random hexamers and dUTP was incorporated in the 2nd strand to retain strand information. Strand-specific RNA-seq (dUTP)

Experiment attributes:

GEO Accession: GSM2735508

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 22.6M spots, 1.7G bases, 715.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR5909303	22,630,380	1.7G	715.8Mb	2017-08-11

ID:: 4355034

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