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SRX3091732: GSM2741665: C. flo early pupae - whole body - rep 1; Camponotus floridanus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 24.3M spots, 4.9G bases, 2.1Gb downloads

Submitted by: NCBI (GEO)
Study: Whole body RNA-seq from developmental stages of Camponotus ants
show Abstracthide Abstract
RNA from Camponotus embryos, instar 1 larvae, instar 4 larvae, early pupae, late pupae (minor and major), adult workers, and males was sequenced to look for gene expression changes throughout development. Overall design: RNA was extracted from the whole body of the ants and sequenced. The RNA-seq was performed for a study that reports PacBio-based reassembly for our two ant genomes. However, those versions of the assemblies are not in a database yet. The records will be updated with the proper links to the assembly records once available.
Sample: C. flo early pupae - whole body - rep 1
SAMN07503302 • SRS2429095 • All experiments • All runs
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA was extracted from full bodies with TRIzol. DNA was removed using Turbo DNase. RNA was fragmented by boiling in presence of divalent cations. RT was performed with random hexamers and dUTP was incorporated in the 2nd strand to retain strand information. Strand-specific RNA-seq (dUTP)
Experiment attributes:
GEO Accession: GSM2741665
Links:
Runs: 1 run, 24.3M spots, 4.9G bases, 2.1Gb
Run# of Spots# of BasesSizePublished
SRR593147424,332,1204.9G2.1Gb2018-05-25

ID:
4381437

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