RNA-Seq of Ricinus communis: Leaves control 2 - SRA

SRX3241212: RNA-Seq of Ricinus communis: Leaves control 2
1 ILLUMINA (Illumina HiSeq 2500) run: 15.3M spots, 3.1G bases, 1,005.9Mb downloads

Design: Total RNA was isolated from 100 mg of the sample, consisting of a mixture of three plants. Two biological replicas were prepared, REP1 and REP2. RNA was extracted with the Rneasy Plant Mini Kit, and > 10 ug were used for each sample. The mRNA was separated with poly-dT beads, then fragmented to an average size of 200 bp and used to prepare cDNA, with random primers to start the first-strand synthesis. cDNA ends were repaired and 3'A was added before ligation of adapters with indexes. A strand-specific protocol was followed. After pre-amplification by PCR, the process of cluster generation and sequencing followed.

Submitted by: UFRJ

Study: Analysis of Castor bean response to drought stress

PRJNA401329 • SRP119313 • All experiments • All runs

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Castor bean (Ricinus communis L.) is one of the main crops with potential for biofuel production, especially due to the high oil content in the total biomass of the seed. Drought limits crop growth and development, significantly reducing production. This study aimed to understand the possible response mechanisms of physiological, biochemical, and molecular processes in castor bean.

Sample: Ricinus communis leaves from control plants

SAMN07602879 • SRS2564309 • All experiments • All runs

Organism: Ricinus communis

Library:

Name: LC_REP2_lib

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 15.3M spots, 3.1G bases, 1,005.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR6128650	15,327,468	3.1G	1,005.9Mb	2018-01-31

ID:: 4557242

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