Sequencing total RNA from infected Micro-Tom roots - SRA

SRX342200: Sequencing total RNA from infected Micro-Tom roots
1 ILLUMINA (Illumina MiSeq) run: 5.9M spots, 3G bases, 1.6Gb downloads

Design: Micro-Toms were grown on sand: clay (60:40) mixture in greenhouse for 14 days and then inoculated with 10,000 reniform nematodes. Roots were collected 30 days post-inoculation (dpi) and were used for isolating total RNAs using mirVana, RNA Isolation procedure (Ambion). The total RNA quality and quantity was assessed using the TapeStation 2200 (Agilent). Library was prepared with ovation method and sequenced on Illumina HiSeq 2000.

Submitted by: J. Craig Venter Institute (JCVI)

Study: Transcriptome Analyses of Reniform Nematode Pathogen Infection

PRJNA217491 • SRP029380 • All experiments • All runs

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This study compares two cotton cultivars (G. hirsutum cv. Fibremax and G. hirsutum cv. Lonren) and a tomato cultivar (S. lycopersicum cv. Micro-Tom) with differing response to Rotylenchulus reniformis (reniform nematode) infection. The purpose of the project was to identify genes and microRNAs that were recognized as being associated with defense or resistance mechanisms in the plants.

Sample: Total RNA from infected Micro-Tom roots

SAMN02338740 • SRS475111 • All experiments • All runs

Organism: Solanum lycopersicum

Library:

Name: Total RNA from roots of infected Micro-Tom

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Spot descriptor:

1 forward102 reverse

Pipeline: show...hide...

Program	Version
Demultiplex Software	vCassava 1.8.0

Runs: 1 run, 5.9M spots, 3G bases, 1.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR960405	5,893,628	3G	1.6Gb	2014-08-30

ID:: 483680

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