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SRX3470834: GSM2888866: Mated queen 1; Solenopsis invicta; RNA-Seq
4 ILLUMINA (Illumina HiSeq 2500) runs: 17.3M spots, 2.2G bases, 883.5Mb downloads

Submitted by: NCBI (GEO)
Study: Fire ant alate virgin and de-alate mated queen brain transcriptomes
show Abstracthide Abstract
Transcriptomes of dissected brains from alate virgin and de-alate mated queens from polygyne fire ants were analyzed and compared. Four replicates of each condition were obtained. Thirteen genes were upregulated in mated queen brain and nine were downregulated. We found that for four differentially expressed genes in brain selected for qPCR analyses, changes in gene expression were most likely driven by the changes in physiological state (i.e. age, nutritional status or dominance rank) or in social environment (released from influence of primer pheromone). Overall design: Fire ant brain mRNA profiles of de-alate mated and alate virgin queens were generated by deep sequencing of four replicates per condition, using Illumina.
Sample: Mated queen 1
SAMN08177032 • SRS2757523 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Brains were disssected in ice-cold PBS , flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq Stranded Total RNA library Prep Kit was used with 1 ug of total RNA RNA libraries were prepared for sequencing using standard Illumina protocols with the Illumina TruSeq Stranded Total RNA library Prep Kit with 1 ug of total RNA
Experiment attributes:
GEO Accession: GSM2888866
Links:
Runs: 4 runs, 17.3M spots, 2.2G bases, 883.5Mb
Run# of Spots# of BasesSizePublished
SRR63759994,297,201537.2M214.1Mb2018-05-10
SRR63760004,305,455538.2M217.7Mb2018-05-10
SRR63760014,302,670537.8M215.2Mb2018-05-10
SRR63760024,399,869550M236.5Mb2018-05-10

ID:
4832398

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