Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Brains were disssected in ice-cold PBS , flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq Stranded Total RNA library Prep Kit was used with 1 ug of total RNA RNA libraries were prepared for sequencing using standard Illumina protocols with the Illumina TruSeq Stranded Total RNA library Prep Kit with 1 ug of total RNA