GSM2891774: B228; Lynx rufus; RNA-Seq - SRA

SRX3478108: GSM2891774: B228; Lynx rufus; RNA-Seq
2 ILLUMINA (Illumina HiSeq 4000) runs: 23.9M spots, 2.4G bases, 847.3Mb downloads

Submitted by: NCBI (GEO)

Study: Genome-wide expression reveals multiple systemic effects of anticoagulant poisons in bobcats (Lynx rufus)

PRJNA422754 • SRP126924 • All experiments • All runs

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We evaluated AR-related effects on genome wide expression patterns in a population of bobcats in southern California. We identify differential expression of genes involved in xenobiotic metabolism, endoplasmic reticulum stress response, epithelial integrity, and both adaptive and innate immune function. Further, we find that differential expression of immune related genes may be attributable to AR-related effects on leukocyte differentiation. Collectively, our results provide an unprecedented understanding of the sublethal effects of AR exposure on a wild carnivore. Overall design: Blood samples from 26 bobcats tested positive for Anticoagulant Rodenticides (Ars) and 26 samples of bobcats not tested positive for ARs.

Sample: B228

SAMN08196012 • SRS2764095 • All experiments • All runs

Organism: Lynx rufus

Library:

Instrument: Illumina HiSeq 4000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA was extracted from 0.5 mL whole blood using the Ambion Mouse RiboPure Blood extraction kit, followed by globin removal using the Ambion GlobinClear Mouse kit (Life Technologies, Inc). RNA was quantified on the Agilent bioanalyzer (Agilent Technologies, USA). Each sample was uniquely tagged with custom index sequences developed at UCLA (Faircloth et al., 2014) comparable to Illumina TruSeq tags. Individual sample libraries were then pooled in equimolar ratios, with 13 or 14 samples per pool and each pool sequenced on two lanes of an Illumina HiSeq 2500 or HiSeq 4000 sequencer

Experiment attributes:

GEO Accession: GSM2891774

Links:

NCBI link: NCBI Entrez (gds)

Runs: 2 runs, 23.9M spots, 2.4G bases, 847.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR6384439	12,720,910	1.3G	432.5Mb	2018-03-26
SRR6384440	11,228,782	1.1G	414.8Mb	2018-03-26

ID:: 4841255

SRA

Sequence Read Archive

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