susceptible Bt fed 12 hr whole rep 2 - SRA

SRX3530848: susceptible Bt fed 12 hr whole rep 2
1 ILLUMINA (Illumina HiSeq 2500) run: 11.2M spots, 2.3G bases, 1.1Gb downloads

Design: RNA was extracted using Trizol and purified by Direct-zol RNA Mini Prep kit (Zymo Research, Irving, CA). DNase treatment (Thermo Fisher, Waltham, MA) was incorporated to remove genomic DNA contamination. Before library preparation all 48 samples were checked for integrity using a fragment analyzer (University of Missouri DNA Core Laboratory). Strand-specific RNA-Seq libraries were constructed using the Illumina TruSeq HT Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA), following the manufacturer s instructions. For whole larvae experiment, a total of 24 libraries (2 insect colonies 2 corn lines 2 time 3 biological replications) were normalized, pooled and sequenced on two lanes of Illumina HiSeq 2000 sequencer using 100-nucleotide pair-end protocol (Global Biologics LLC, Columbia, MO, USA).

Submitted by: USDA-ARS

Study: Diabrotica virgifera virgifera strain:eCry3.1Ab resistant Transcriptome or Gene expression

PRJNA416935 • SRP127188 • All experiments • All runs

show Abstracthide Abstract

Larval western corn rootworm toxicogenomic response to Bt intoxication.

Sample: susceptible Bt fed 12 hr whole rep 2

SAMN07969493 • SRS2771191 • All experiments • All runs

Organism: Diabrotica virgifera virgifera

Library:

Name: ESBW12Rep2

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq