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SRX353982: GSM1234670: SD_G7_T5_C; Glycine soja; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 9.2M spots, 915.3M bases, 624.2Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Transcriptome analysis of photoperiodic flowering in soybean.
show Abstracthide Abstract
In order to identify candidate genes that are involved in soybean flowering transition in response to photoperiods, we performed RNA sequencing analysis under different photoperiod treatments. We identified genes exhibiting daily oscillation patterns under different photoperiod treatments, genes under control of maturity loci (E1, E2, E3 and E5), and genes that constitute the soybean flowering gene network. Overall design: RNA sequencing analysis was performed using Williams 82, Clark and four NILs in Clark that are polymorphic for E loci (E1, E2, E3 and E5), as well as the wild soybean Glycine soja, under different photoperiod treatments.
Sample: SD_G7_T5_C
SAMN02358920 • SRS482241 • All experiments • All runs
Organism: Glycine soja
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Plant samples were ground and RNAs were extracted using the RNeasy® Plant Mini Kit (Qiagen®). The RNAseq libraries were prepared with Illumina's 'TruSeq RNAseq Sample Prep kit. The libraries were pooled and quantitated by qPCR, and sequenced for 101 cycles on a HiSeq2000 using a TruSeq SBS sequencing kit version 3 and analyzed with Casava-1.8 (pipeline 1.9), following the manufacturer's instructions (Illumina, San Diego, CA).
Experiment attributes:
GEO Accession: GSM1234670
Links:
External link:
Runs: 1 run, 9.2M spots, 915.3M bases, 624.2Mb
Run# of Spots# of BasesSizePublished
SRR9938759,152,754915.3M624.2Mb2017-07-21

ID:
502209

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