RNA-Seq of Rana temporaria: toe clip: Poole, UK - SRA

SRX3603947: RNA-Seq of Rana temporaria: toe clip: Poole, UK
1 ILLUMINA (Illumina HiSeq 2000) run: 127.7M spots, 24.5G bases, 10Gb downloads

Design: RNA was extracted from toe clips collected from wild caught R. temporaria. RNA from 3 males and 3 females with RNA integrity scores higher than 8 was pooled. Reverse transcription was conducted using a combination of random hexamer and oligo DT primers. Libraries were sequenced on a HiSeq2000 using v3 chemistry.

Submitted by: University of Exeter

Study: RNA-Sequencing data from wild caught European common frog (R. temporaria) populations both with and without a history of ranaviral disease

PRJNA431814 • SRP131529 • All experiments • All runs

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Ranaviruses are globally distributed and can cause mass mortality within amphibian populations. As such they are of great conservation concern. This project constructed a de-novo transcriptome of the European common frog (R. temporaria) and used it to compare gene expression levels between three populations of R. temporaria with a positive history of ranaviral disease outbreaks and three populations that have remained disease free.

Sample: Frogs sampled in Poole, Dorset, UK.

SAMN08407979 • SRS2871877 • All experiments • All runs

Organism: Rana temporaria

Library:

Name: POOLE_RNA-Seq

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 127.7M spots, 24.5G bases, 10Gb

Run	# of Spots	# of Bases	Size	Published
SRR6515976	127,710,961	24.5G	10Gb	2018-01-26

ID:: 4997739

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