Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNeasy plant minikit (Qiagen) TruSeq Stranded mRNA Sample Preparation Protocol (Illumina) Equimolar amounts of libraries were pooled and diluted towards 6pM concentrations for paired end clustering on one lanes of a flow cell V4 (Illumina) and analyzed paired end on a HiSeq2500 instrument using a 126,7,126 flow cycle pattern.