SEP - SRA - NCBI

SRX4225891: SEP
1 ILLUMINA (Illumina HiSeq 2000) run: 29.4M spots, 5.9G bases, 3.8Gb downloads

Design: QC of sample RNA was done by Agarose Gel electrophoresis, Nanodrop and Agilent 2100 and library After QC, mRNA of Eukaryote organisms is enriched from total RNA by Oligo (dT) beads and the Ribo-Zero kit is used to remove rRNA from the Prokayote organisms. First, the mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment followed by QC of library using Qubit 2.0, Agilent 2100 and Q-PCR

Submitted by: University of Florida

Study: Transcriptome characterization of Impatiens for downy mildew resistance

PRJNA475555 • SRP150717 • All experiments • All runs

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Transcriptome sequencing if Impatiens to characterize the features of RNA of Impatiens

Sample: Leaf sample from Impatiens walleriana cultivar Super Elfin XP Pink for RNAseq

SAMN09393910 • SRS3425190 • All experiments • All runs

Organism: Impatiens walleriana

Library:

Name: SEP

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq