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SRX4408097: GSM3291614: 01_03_06_control group; Trachemys scripta elegans; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 25.3M spots, 7.6G bases, 2.8Gb downloads

Submitted by: NCBI (GEO)
Study: Transcriptomes and molecular pathway analysis of the liver in the red-eared slider turtle Trachemys scripta elegans under chronic salinity stress
PRJNA481871 • SRP154424 • All experiments • All runs
show Abstracthide Abstract
In order to understand the molecular mechanisms of salinity adaptation, high-throughput RNA-Seq was utilized to discover the gene expression profiles and pathways that responded to elevated salinity in the liver of T. s. elegans Overall design: Three turtles from each group were divided into three groups exposed to 0, 5, or 15 psu (practical salinity units) for 30 days, and sampled following a 24 h fast and anesthetized by storing at -20? cryo-anesthesia for 0.5-1 h.
Sample: 01_03_06_control group
SAMN09691331 • SRS3561774 • All experiments • All runs
Organism: Trachemys scripta elegans
Library:
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Extraction of total RNA from liver samples used TRIzol® Reagent, following manufacturer's instructions. Total RNA purity and concentration were determined using a NanoDrop 2000. RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM3291614
Links:
Runs: 1 run, 25.3M spots, 7.6G bases, 2.8Gb
Run# of Spots# of BasesSizePublished
SRR754056925,340,6997.6G2.8Gb2018-11-18

ID:
5990059

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