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SRX462319: Comprehensive transcriptome analysis of Bemisia tabaci from India
3 ILLUMINA (Illumina HiSeq 1000) runs: 135.8M spots, 27.4G bases, 16.7Gb downloads

Design: Total RNA was isolated from each sample separately using Tri reagent (Sigma, USA) following the manufacturers protocol. DNA contamination in the RNA preparations was removed using DNA-free Kit (Ambion, USA). Integrity of RNA samples were analyzed on 2100 Bioanalyzer (Agilent Technologies, USA). Equal amount (5 µg) of total RNA from each sample was pooled, and mRNA was purified using polyT oligo beads. mRNA was fragmented and cDNA library preparation was performed using ‘Tru Seq RNA sample preparation kit v2 from Illumina following the manufacturer specified protocols (http://www.illumina.com/systems /hiseqsystems/hiseq_2000_1000/kits.ilmn).
Submitted by: National Agri-Food Biotechnology Institute
Study: Comprehensive transcriptome analysis of Bemisia tabaci from India
PRJNA237273 • SRP036178 • All experiments • All runs
Sample: Model organism or animal sample for Bemisia tabaci
SAMN02617556 • SRS551368 • All experiments • All runs
Organism: Bemisia tabaci
Library:
Name: Bemisia tabaci total RNA
Instrument: Illumina HiSeq 1000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: unspecified
Layout: PAIRED
Spot descriptor:
1  forward102  reverse

Runs: 3 runs, 135.8M spots, 27.4G bases, 16.7Gb
Run# of Spots# of BasesSizePublished
SRR115920841,914,4338.5G5.2Gb2015-02-04
SRR115920948,048,6839.7G5.9Gb2015-02-04
SRR115921045,840,5619.3G5.7Gb2015-02-04

ID:
642487

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