SRA

To generate a complete neurotranscriptome of the Liverpool strain of Aedes aegypti, we performed Illumina RNA-sequencing (RNAseq) on 10 tissues from pools of female mosquitoes and 7 tissues from pools of male mosquitoes. These tissues include the brain, peripheral sensory tissues and organs (antennae, maxillary palp, proboscis, ovipositor/abdominal tip, legs, and ovary) and were replicated at least 3 times for each condition. Furthermore, to understand the influence of blood-feeding state on gene expression, we performed RNA-seq on a subset of tissues in female mosquitoes at three time-points: prior to a blood-meal (sugar-fed), at 48 hours following a blood-meal (blood-fed), and at 96 hours following a blood-meal (ovipositing). These experiments allowed us to characterize gene expression at high resolution in specific tissues, identify sexually-dimorphic gene expression, and to identify gene expression in female tissues that is regulated by blood-feeding state.