SRX4742935: piD4 GFP cell
1 ILLUMINA (HiSeq X Ten) run: 13.7M spots, 4.1G bases, 1.6Gb downloads
Design: monkey blastocysts after injection of pESCs were cultured on 8-well IbiTreat -plates (IB-80826; Ibidi GmbH) with in vitro culture media in 5% CO2 incubator. For the first 48 h the embryos were cultured in in vitro culture medium 1 (IVC1). On in vitro culture day 2 (embryo day 7 post fertilization), 50% of the culture medium was replaced with in vitro culture medium 2 (IVC2). From in vitro culture day 3 on, IVC2 medium was used and medium was refreshed on a daily basis. For the 96 h, the GFP single cell were isolated. Embryos were digested into single cells following treatment with 0.5% Trypsin. Cells were grouped with GFP negative or positive using inverted fluorescence microscope. GFP+ injected cells and GFP- host embryonic cells were collected for gene expression analysis, respectively. To exclude GFP cell marker expression used in PCR may come from recipient embryonic cells, single GFP+ cells were picked up under fluorescence microscopy, respectively. The contaminations of the host embryonic cells were excluded before performing RNA isolation. cDNAs synthesis and amplification were performed with Discover-sc WTA Kit V2 (Vazyme N711, China) according to the instruction manual. The qualities of the cDNAs were evaluated by Bioanalyzer 2100.
Submitted by: Kunming University of Science and Technology
Sample:
monkey blastocysts after injection of pESCs were cultured on 8-well IbiTreat -plates (IB-80826; Ibidi GmbH) with in vitro culture media in 5% CO2 incubator. For the first 48 h the embryos were cultured in in vitro culture medium 1 (IVC1). On in vitro culture day 2 (embryo day 7 post fertilization), 50% of the culture medium was replaced with in vitro culture medium 2 (IVC2). From in vitro culture day 3 on, IVC2 medium was used and medium was refreshed on a daily basis. For the 96 h, the GFP single cell were isolated.Library:
Name: piD4_pESC-GFP5
Instrument: HiSeq X Ten
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Runs:
1 run, 13.7M spots, 4.1G bases, 1.6Gb