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SRX498113: GSM1356024: Rex rabbit, back skin, 42 days, white color(W); Oryctolagus cuniculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 26.9M spots, 5.4G bases, 3.6Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Analysis of genes that control fur color formation in domestic rabbits using Solexa sequencing
show Abstracthide Abstract
We used Solexa sequencing technology to probe the gene expression of dorsal skin tissues from three full-sib Rex rabbits of different colors. The number of expressed genes in each sample was approximately 14,700. Compared with the chinchilla Rex rabbit control sample, the numbers of genes that exhibited greater than a 2-fold change in expression with a false discovery rate of =0.001 in the black rabbit sample and white rabbit sample were 1,809 and 460, respectively, and the number of common differentially expressed genes was 257.Of the 257 genes, 32 genes that were upregulated in sample B and downregulated in sample W. The FABP7 gene was the only gene that was downregulated in the B sample and upregulated in the W sample Overall design: The samples collected with three full-sib individual and they were raised under the same conditions for 42 days(at the first molt). The fur colors of them were characterized as black (B), white (W), and chinchilla (C).
Sample: Rex rabbit, back skin, 42 days, white color(W)
SAMN02693836 • SRS580013 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted from the back skin of the Rex rabbits using TRIzol reagent (Invitrogen, USA). A Qubit fluorometer (Invitrogen, USA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., USA) were used to determine the quality and quantity of the RNA. The mRNA was enriched using oligo (dT) magnetic beads, then fragmented into short fragments (approximately 200-700 nt) using fragmentation buffer (Invitrogen, USA). Double-stranded cDNA product was synthetized and purified . RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM1356024
Links:
External link:
Runs: 1 run, 26.9M spots, 5.4G bases, 3.6Gb
Run# of Spots# of BasesSizePublished
SRR120125626,883,9825.4G3.6Gb2014-12-31

ID:
688210

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