Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA was extracted from the back skin of the Rex rabbits using TRIzol reagent (Invitrogen, USA). A Qubit fluorometer (Invitrogen, USA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., USA) were used to determine the quality and quantity of the RNA. The mRNA was enriched using oligo (dT) magnetic beads, then fragmented into short fragments (approximately 200-700 nt) using fragmentation buffer (Invitrogen, USA). Double-stranded cDNA product was synthetized and purified . RNA libraries were prepared for sequencing using standard Illumina protocols