GSM1361892: F2 single-end; Oryza sativa Japonica Group; RNA-Seq - SRA

SRX507921: GSM1361892: F2 single-end; Oryza sativa Japonica Group; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 45.9M spots, 4.6G bases, 2.8Gb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: Transcriptome profiling of various organs at different developmental stages in rice (single-end)

PRJNA243371 • SRP040813 • All experiments • All runs

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To identificate long noncoding RNAs in rice, we profiled transcriptome of various organs at different developmental stages using stranded single-end RNA-seq based on poly(A) selection. Overall design: Transcriptom profiling in flower buds, flowers, flag leaves and roots sampled before flowering and after flowering, milk grains and mature seeds.

Sample: F2 single-end

SAMN02716061 • SRS586830 • All experiments • All runs

Organism: Oryza sativa Japonica Group

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: RNA was extracted using the Qiagen RNeasy Plant Mini kit. Total RNA was treated with Turbo DNase I (Life Technologies AM2238) according to product specification. DNase I treated RNA was then applied again to an RNeasy spin column with 0.5 volumes of ethanol, washed and eluted according to the manufacturer’s instructions. Sequencing libraries were prepared using the Illumina Stranded mRNA sample Prep kit, set A (RS-122-2101, Illumina Inc.) according to the manufacturer's instructions. The quality and size of cDNA libraries for sequencing were checked using the Agilent 2200 TapeStation system (Agilent Inc.). The libraries were sequenced for 100 cycles on HiseqTM 2500 (Illumina Inc.)

Experiment attributes:

GEO Accession: GSM1361892

Links:

External link: GEO Sample GSM1361892

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 45.9M spots, 4.6G bases, 2.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR1213691	45,875,792	4.6G	2.8Gb	2015-11-27

ID:: 707225

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